Webthe GIY-YIG family enzymes are functional as monomers. We show here that the Cfr42I restriction endonuclease which belongs to the GIY-YIG family and recognizes the … WebMar 15, 2000 · Less is known about the GIY-YIG ORFs which constitute another family of endonucleases. In this paper we describe the presence of one optional GIY-YIG ORF in the second intron of the mitochondrial cytochrome b gene in the fungus Podospora curvicolla.

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WebI-TevI, a member of the GIY-YIG family of homing endonucleases, consists of an N-terminal catalytic domain and a C-terminal DNA-binding domain joined by a flexible linker. The GIY-YIG motif is in the N-terminal domain of I-TevI, which corresponds to a phylogenetically widespread catalytic cartridge that is often associated with mobile genetic ... WebMany mortals stray into Il Mheg─mortals that could grant us patronage. But they seldom make it past Lydha Lran because the pixies turn them into leafmen first! kleens theorem automata

The GIY‐YIG endonuclease domain of Arabidopsis MutS …

WebFeb 1, 2006 · This analysis allowed for extending the GIY-YIG superfamily to include members of COG3680 and a number of proteins not classified in COGs and to predict that these proteins may function as... WebJan 1, 2014 · (PDF) Evolutionary adaptation of the GIY-YIG domain for specific DNA binding in I-CreII Home Biomolecules DNA Biological Science Nucleic Acids Molecular Biology DNA Binding Evolutionary... WebThe GIY-YIG endonuclease domain. MI, USA) containing 50 lg mL 1 ampicillin and 30 lg mL 1 chloramphenicol was inoculated with 5 mL of the overnight culture. After 3 h of cultivation at 37 °C, iso-propyl b-D-thiogalactopyranoside was added to a final con-centration of 0.1 mM. The cells were cultivated at 37 °C for kleenmaid washing machines spare parts