Cytotoxicity of pei
WebJun 1, 2005 · PEI is also cytotoxic, but the molecular basis of its cytotoxicity is poorly understood. Here, we have demonstrated that branched (25 kDa) and linear (750 kDa) PEI can both induce membrane damage and initiate apoptosis in three clinically relevant … Web1 day ago · Binary and ternary polyplexes with PEI/DNA ratio of 1:1 (w:w), as well as free PEI at an equivalent concentration, exhibited minimal cytotoxicity, regardless of the presence of PGA. Free PEI at concentrations equivalent to that present in the polyplexes with PEI/DNA 3:1 and 5:1 (w:w), however, decreased viability by 20–30% and ∼40–60% ...
Cytotoxicity of pei
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WebDec 1, 2024 · The cytotoxicity and the potential of PEI_ Ti 3 C 2 were examined on triple-negative breast cancer cell model MDAMB-231 cells. The cells were seeded on 96 well plates and incubated with PEI_ Ti 3 C 2 (25–100 µg mL −1) for 24 h. The cells were washed and irradiated with 808 nm laser for 10 min. WebMay 25, 2024 · Cytotoxicity of PEI, WSLP, and of WSLP/pDNA is evaluated on human A549 and HeLa cells. A remarkable dependence of the toxicity on the dose, cholesterylation, and charge ratio is detected. Transfection is monitored by flow cytometry and by fluorescence microscopy.
WebThe different PEI compositions provide various properties and reactivities to particular structures. 21 In addition, the molecular weight of PEI varies broadly, and high-molecular-weight-PEI is associated with increased cytotoxicity. Also, the effects of PEI on DNA damage are considered based on the polymer/DNA (C/P) ratios. WebCell culture and cytotoxicity assay SH-SY5Y human neuroblastoma cells (C611, NCBI, Tehran, Iran) were incubated at 37°C in a humidified (100% humidity) atmosphere of 5% CO 2 in 100 μL DMEM (1 g/L glucose, 2 mM glutamine) supplemented with 10% FBS, 100 μg/mL streptomycin, and 100 IU/mL penicillin.
WebHowever, PEI, especially the branched one, is much toxic due to the presence of amino group as well as cationic nature. Hence, there is a need to further minimize its cytotoxicity by making its derivatives with polymers having biocompatible nature. WebSep 6, 2024 · Cytotoxicity of GO-PEI were investigated by calculating the percent of cell survival by MTT assay. MTT data analyzed in excel. Researchers, who want to research …
WebPoly(ethylenimine) (PEI) is a cationic macromolecule commonly used in gene transfer/therapy protocols with high transfection efficiency both in vitro and in vivo. PEI is also cytotoxic, but the molecular basis of its …
WebJun 30, 2015 · Therefore, it seems that PEI could induce cell death through induction of apoptosis process. 21 The purpose of this study was to separate and recognize the … flashcards sobre abdome obstrutivoWebSep 24, 2012 · The transfection efficiency and the cytotoxicity of PEI are closely linked to its structural activity. This structural relationship is an essential issue to be investigated in detail in order to show the effect of chemical structure, hydrophobicity, charge density, and molecular weight of PEI on its transfection activity as well as cytotoxicity flashcards significatoWebNov 8, 2002 · To determine whether the lower expression of PEI-complexed DNA at the N/P ratio of 20/1 than the 10/1 N/P ratio is due to the cytotoxicity of PEI, we performed the MTT assay for cell viability. flashcards simpson familyWebOct 13, 2024 · We revealed that the toxicity of LPEI was due to oxidative stress resulting from the cellular uptake of LPEI. To suppress the toxicity of LPEI, an antioxidant, 2,2,6,6 … flashcards sjabloonWebOct 8, 2007 · The synthesized reducible PEI show reduced cytotoxicity and comparable transfection activity when compared with control non-reducible PEI. The synthesized reducible PEI represent better defined polymers in comparison with earlier attempts to prepare reducible PEI by random crosslinking of PEI oligomers [11, 12]. flash cards sizeWebPEI is also cytotoxic, but the molecular basis of its cytotoxicity is poorly understood. Here, we have demonstrated that branched (25 kDa) and … flashcards smileysWebPolyethylenimines (PEIs) are outstanding macromolecules belonging to the polycations used in gene transfection. The transfection efficiency and cytotoxicity of PEIs increase with the increase in their molecular weight. flash cards sight words for kindergarten