WebJul 11, 2024 · Brefeldin A v Monensin: How to Hunt for Proteins. Published July 11, 2024. Posted in: Protein Expression and Analysis. As any good biologist knows, one of the … Or register your account here WebJun 15, 1992 · Monensin and brefeldin A differentially affect the phosphorylation and sulfation of secretory proteins Authors P Rosa 1 , S Mantovani , R Rosboch , W B Huttner Affiliation 1 Consiglio Nazionale delle Ricerche-Center of Cytopharmacology, Department of Pharmacology, Milan, Italy. PMID: 1601888 Abstract
(PDF) Detection of T-Cell Degranulation: CD107a and b
WebApr 10, 2024 · PBMC were expanded in vitro as previously described.[13] Expanded PBMCs were incubated with pooled peptides at a final concentration of 10 μg/mL for 1 h, and then with brefeldin A and monensin for an additional 5 h. Dead cells were labeled using BD Horizon Fixable Viability Stain 510 (BD Biosciences). WebOct 31, 2012 · Brefeldin A, but not monensin, enables flow cytometric detection of interleukin-4 within peripheral T cells responding to ex vivo stimulation with Chlamydia … manucharyan thesis
Brefeldin A (BFA) Reversible protein transport inhibitor Hello Bio
Webbrefeldin A A macrocyclic lactone synthesised from palmitic acid by several fungi, which has antiviral activity. Immunology Brefeldin A inhibits exocytosis and antigen processing … WebBrefeldin A (BFA) is a fungal macrocyclic lactone and a potent, reversible inhibitor of intracellular vesicle formation and protein trafficking between the endoplasmic reticulum (ER) and the Golgi apparatus [1]. BFA and its analogs are promising inhibitors in drug development due to a number of key features such as apoptosis‑inducing ... WebAnd because the difference between groups are so clear so I just use Brefeldin A but not PMA, Ionomycin for 4 hours. ... Some resources suggest to use inhibitors like monensin to inhibit the ... manuchasse site officiel